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Fig. 2 | BMC Bioinformatics

Fig. 2

From: High quality genome assemblies of Mycoplasma bovis using a taxon-specific Bonito basecaller for MinION and Flongle long-read nanopore sequencing

Fig. 2

Validation of the specificity of taxon-specific custom-pg45 Bonito basecalling using a E. coli ATCC 25922 and b nine additional M. bovis field strains. a Performance of the M. bovis PG45 custom-trained Bonito model was tested in comparison with E. coli ATCC 25922, only showing taxon-specific superior performance. Dotted lines represent predicted gene numbers in M. bovis PG45 and E. coli ATCC 25922 reference genomes, respectively. b Extrapolation of the custom-pg45 implementation to nine additional M. bovis field strains, shows overall increased performance for all strains in comparison to default Guppy basecalling. As a matter of validation of the UniCycler consensus genomes as “true” references, the M. bovis PG45 (NC_014760.1) was included in the analysis and indicated in grey. A completeness of 100% indicates all genomic markers (n = 226 and n = 1212 for Mycoplasma spp. and Escherichia spp., respectively) were present. A 100% Genome Fraction indicates the full UniCycler MiSeq M. bovis field strain genome was covered

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BMC Bioinformatics

ISSN: 1471-2105

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