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Fig. 3 | BMC Bioinformatics

Fig. 3

From: High quality genome assemblies of Mycoplasma bovis using a taxon-specific Bonito basecaller for MinION and Flongle long-read nanopore sequencing

Fig. 3

Comparative analysis for the implementation of cheaper single-use Flongle flow cells, using the custom-pg45 model. a Overall Flongle consensus accuracy Q-scores are lower as compared to the MinION sequencing platform. This is also reflected in lowered genome completeness and predicted gene numbers. b In-depth analyses of decreased Flongle performance is suggested to be related to increased numbers of indels (per 100 kbp), showing little differences in insertion Q-score, though a decrease of accuracy for reported deletions is observed. A completeness of 100% indicates all genomic markers (n = 226 for Mycoplasma spp.) were present. A 100% Genome Fraction indicates the full UniCycler MiSeq M. bovis field strain genome was covered

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