Fig. 3
From: TruNeo: an integrated pipeline improves personalized true tumor neoantigen identification
The filtering process of personalized neoantigens for patient 01 by TruNeo software. We applied WES and RNA-Seq sequencing to a patient with advanced squamous cell carcinoma. 451 somatic mutations were detected. 313 of them were non-silent mutations. We predicated 254 neoantigens by TruNeo software. 116 neoantigens could be expressed. Top 10 MHC class I neoantigen were ranked by TruNeo software, and 5 of the top 10 neoantigens were validated by Elispot assay