Fig. 11
From: Auto3DCryoMap: an automated particle alignment approach for 3D cryo-EM density map reconstruction
Fully automated perfect 2D side-view particle alignment results using the KLH dataset [12] and the intensity-based image registration. a, b Two localized aligned particle images that are not perfectly aligned. c Two-particle image projection (a, b). d Default image alignment (initial registration). e Optimizer adjustment and metric configuration-based image registration. f Image registration based increasing the maximum iteration number. g Image registration-based optimization and rigid transformation. h Image remigration using affine transform