Fig. 1

Head region detection in oriented zebrafish larvae using single template matching. a Searched image (2048 × 2048 pixels, scale bar: 1 mm) with template as inset (188 × 194 pixels), search region in orange (1820 × 452 pixels) and predicted location in blue. The red cross corresponds to the position of the global maximum of the correlation map (as in b). b Correlation map with global maximum (red cross) indicating the position of the bounding box in a. The grid area indicates the smaller size of the correlation map compared to the image in which the search is performed (see also Additional file 13). c Montage of detected head regions within a 96 well plate