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Fig. 2 | BMC Bioinformatics

Fig. 2

From: A novel virtual barcode strategy for accurate panel-wide variant calling in circulating tumor DNA

Fig. 2

Comparison between the virtual tag and real tag using 3 Oncosmart2 UMI samples. a Mean family numbers and corresponding SD from 10 samples (20,000 random genomic positions per sample) obtained using the UMI alone (blue bar), the real tag (orange bar) and the virtual tag (red bar). b Significant linear relationship between virtual family numbers and real family numbers for 20,000 genomic positions (R2 = 1.0). c Recovery rate distribution for real family numbers at 20,000 genomic positions. d Percentage of wrongly clustered virtual family calculated by different assigned numbers of real family. e Comparison between f = 1.0 virtual family numbers (orange bar) and f = 1.0 real family numbers (blue bar) among six positive sites in one UMI sample with an AF level of 0.1%. f Mean fraction and corresponding SD of the panel-wide error-free position before (green bar) and after application of the real tag (blue bar) and the virtual tag (red bar) in 3 UMI samples

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