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Fig. 2 | BMC Bioinformatics

Fig. 2

From: Prediction model construction of mouse stem cell pluripotency using CpG and non-CpG DNA methylation markers

Fig. 2

Pseudo-time of single ESCs based on gene expression. a Two-dimension plot of 75 single cells based on gene expression data, applying monocle2. Color bar represents cell pseudo-time based on gene expression data. b A violin plot of pseudo-times of cells according to cell culture environment. Red dots represent pseudo-time for cells grown in 2i media; blue dots represent cells cultured in serum. p represents a result of ranksum test between cells in 2i and serum environments by Wilcoxon rank-sum test. c A heat map of gene expression ordered using the monocle2 cell ordering method. Pluripotent/differentiation marker genes are sorted according to Spearman correlation coefficients between cell pseudo-time and gene expression levels. Samples are sorted by cell pseudo-time detected by the monocle2 method. Numbers in the right columns indicate Spearman’s correlation coefficients; top red-yellow color bar represents the cell pseudo-time; and the bottom blue/red color bar indicates the environment for cell growth

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