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Table 3 DNA composition and identity analysis for the reference (Ref) and assembled (Ass) DNA molecules

From: Modeling of shotgun sequencing of DNA plasmids using experimental and theoretical approaches

Parameter

DNA

TOPO**

QE**

LEXSY***

Ref

Ass

Ref

Ass

Ref

Ass

GC, %

51.7

52.44

48.32

46.96

58.3

58.51

AT, %

48.3

47.56

51.68

53.04

41.7

41.49

MW, kDa

1239.67

1470.74

1485.06

1767.37

710.69

915.77

Identity

0.54

0.62

0.78

  1. *-pCR™4-TOPO®; **- pQE-30-UA-mCHERRY-GFP; ***- pLEXSY-Ig-1; MW- molecular weight calculated for a single stranded DNA