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Fig. 2 | BMC Bioinformatics

Fig. 2

From: Automated image analysis system for studying cardiotoxicity in human pluripotent stem cell-Derived cardiomyocytes

Fig. 2

Image analysis pipeline and segmentation results. a Image analysis pipeline for segmentation and quantification of the individual cardiomyocytes from the image with α-actinin and DAPI staining. b A sample of segmentation results (segmentation lines: Green; α-actinin signal: Red; DAPI signal: blue). c Comparison of cell masking methods on a typical cluster of cells. Left, original image; middle, binary mask of Otsu thresholding method; right, binary mask of Fuzzy c-mean clustering method. d Comparison of different segmentation methods. The difference of the two methods are the usage of different cell masking method. Left, Propagation result with Otsu cell masking; right, our propagation result with FCM cell masking

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