Fig. 1
From: YSMR: a video tracking and analysis program for bacterial motility
From original frame to final detection. a: the original frame from the video file. b: the frame is converted to grey scale. c: a Gaussian blur with a 3 by 3 kernel is applied. d: the adaptive threshold is applied, leaving white areas as potential bacteria. e: a second, higher, adaptive threshold is applied to generate markers. f: white areas from d which contain markers from E are used as outlines. g: each area is encased in a rectangle and assigned a unique ID, displayed on the original frame