Fig. 1From: DeSignate: detecting signature characters in gene sequence alignments for taxon diagnosesWorkflow of DeSignate. The data analysis commences with the selection of input data, viz., a sequence alignment and (optionally) the corresponding phylogenetic tree. Next, the query and reference groups are selected in the tree or via comma-separated lists, and the search parameters (e.g., size of k-window) are specified. The algorithm ranks all positions in the alignment and identifies both individual and combined candidate characters based on the discriminative power, the query rank, and the reference rank of the respective character state vectors. As proposed by Hadziavdic et al. [16], the entropy plot displays variable (peaks) and conserved regions in the alignmentBack to article page