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Fig. 3 | BMC Bioinformatics

Fig. 3

From: ICEKAT: an interactive online tool for calculating initial rates from continuous enzyme kinetic traces

Fig. 3

EC50/IC50 and high-throughput screening modes. a Plot of a representative IC50 model fit of initial rates. b Widgets for choosing advanced EC50/IC50 analysis settings allow users to convert the x-axis to Log10 scale and fix regression parameters. The data table displays fit values with errors propagated from the initial rate fits for the 4-parameter logistic model. c Plot displaying HTS analysis of initial rates from a representative 96-well plate. d The data table displays initial rates and associated errors for all samples uploaded and highlights cells corresponding to samples with initial rates above (red) or below (blue) the standard deviation threshold defined by the slider (here set to 1 standard deviation from the mean initial rate)

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