Fig. 1

Overview of TAMA. In the read preprocessing step, low-quality input metagenome reads (single- or paired-end) are eliminated. Integrated database with identical set of reference genomes is also created. Initial taxonomy classification results, which has assigned taxon IDs for each read sequence, are generated by using CLARK, Kraken, and Centrifuge with the integrated database. In the meta-analysis process, results from the three tools are calibrated and integrated to produce a read classification and relative species abundance profile. The relative species abundance profile is generated only when the target taxonomic rank is species