Fig. 2

Implementation of ViR with WGS data from Ae. albopictus. a ViR was run with WGS data generated from DNA of both single and pool DNA samples using two assemblies, AaloF1 and AalbF2 [27, 28] and a custom database of viral genomes (Additional file 1). A list of chimeric pairs, indicative of potential integration sites, were obtained for each sample running Vy-PER [5]. Running Module 1 using the chimeric reads identified by Vy-PER resulted in a total of seven potential viral integrations. b Scheme of potential viral integrations and their molecular validation. Each lane is the result of a PCR on mosquito genomic DNA with primers that were designed to check the left or right integration sites. ‘+’ indicates the presence of the viral integration and ‘−’ the absence. c Identification of exact integration sites of nrEVEnew-7 running ViR_LTFinder; d ViR_LTFinder was run with WGS data and the viral sequences of nrEVEnew-4 and nrEVEnew-6 showing that they correspond respectively to the right and left regions of the same integration, which was molecularly validated. ‘+’ indicates the presence of the viral integration and ‘−’ the absence