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Fig. 6 | BMC Bioinformatics

Fig. 6

From: Statistical image processing quantifies the changes in cytoplasmic texture associated with aging in Caenorhabditis elegans oocytes

Fig. 6

Day 3-fied images created from Day 1 images. a Comparison of cytoplasmic texture between Day 1 and Day 3 oocytes. Top, oocyte images; yellow areas indicate representative extracted areas. Scale bar 20 μm. Bottom, images of the extracted areas. The extracted area of the Day 3 oocyte (middle) appears to have a smoother texture than that of the Day 1 oocyte (left). The extracted area of the Day 3 oocyte (right) contains large structures (marked by a dotted white line), whereas that of the Day 1 oocyte is homogeneous. b Extracted areas of a Day 1 image and three Day 3-fied images (left, Smoothed pattern; middle, Large Structure pattern; right, Combination pattern) created from the Day 1 image. The dotted white line indicates the filtered area. c Mm Values (3 × 3-pixel window) of actual Day 1 and actual Day 3 images, and the three patterns of Day 3-fied images. The Smoothed pattern parameter offset was set to 50, 100, and 200. The Large Structure pattern parameter iteration was set to 1, 3, and 5. The Combination pattern parameters (offset, iteration) were set to (50, 1), (50, 3), and (50, 5). Circles indicate the Mm Values of individual animals (n = 10 animals in each age group; orientation of the worms is 0°); red bars indicate the mean values. Error bars indicate SEM. Asterisks indicate statistical significance (Tukey–Kramer test) between actual Day 1 and actual Day 3 images or actual Day 1 and Day 3-fied images (**P < 0.01); N.S., no significant difference between actual Day 3 and Day 3-fied images. d, e Curves of mean COR as a function of distance d when θ = 135 are shown for actual Day 1, actual Day 3, and Day 3-fied oocyte images created with d Smoothed pattern (offset = 50 or 200) or e Large Structure pattern (iteration = 1 or 5). Data are means ± SEM (n = 10 animals in each age group; orientation of the worms is 0°)

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