Fig. 8

Measurement of the size of cytoplasmic granules in DIC images. a Representative DIC images of oocytes. Left, Day 1 oocyte. Right, Day 3 oocyte. Top, Raw images. Bottom, Manual detection of granules on raw images. b Comparison of the size of granules manually measured in Day 1 and Day 3 oocytes. The sizes of many of the elongated and clustered granules in Day 3 oocytes were not measured. Circles indicate individual animals (n = 8 animals in each age group); red bars indicate the mean value. Error bars indicate SEM. Asterisks indicate statistical significance (**P < 0.01; Welch’s two-tailed t test)