Fig. 1From: barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detectionOverview of barcode design and algorithm work flow. a Barcodes consist of two synthetic primer binding sites, a probe annealing site, and two spacer regions. Spacer regions can be adjusted to match the overall G+C content of the organism to be barcoded. b barCoder algorithm work flowBack to article page