Fig. 2

Representative qPCR assay standard curves. Curves were generated using a barcode Btk1 in the pIDTSMART-AMP plasmid backbone, b barcode Btk1 inserted into the B. thuringiensis kurstaki chromosome, c barcode Btk1 inserted into the B. anthracis Sterne chromosome, and d barcode Yp1 inserted into the Y. pestis CO92 pgm⁻ chromosome. For each standard curve, data from three replicates and a trendline are shown. Standard curves for the remaining barcodes in the plasmid backbone are shown in Figure S1 in Additional file 1