Fig. 3From: barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detectionCross-reactivity of the 12 Btk qPCR assays against the 12 Btk barcodes. For each qPCR reaction, 10–12 g (~ 450,000 copies) of the pIDTSMART-AMP plasmid backbone containing the Btk barcode indicated was used as DNA template. Threshold cycle (Ct) values shown are the median of three replicates; ND, Not determinableBack to article page