Table 4 Cross-reactivity of the barcode Btk1 qPCR assay against a panel of potential pathogens and environmental contaminants
From: barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection
DNA templatea | Ctb |
|---|---|
Bacillus anthracis Ames35 | ND |
Bacillus anthracis Sterne 34F2 | ND |
Bacillus cereus Gibson 971 | ND |
Bacillus licheniformis Gibson 46 | ND |
Bacillus megaterium Ford 19 | ND |
Bacillus sphaericus Ford 25 | ND |
Bacillus subtilis subsp. subtilis 168 | ND |
Bacillus thuringiensis serovar kurstaki HD-1 | ND |
Bacillus thuringiensis subsp. konkukian 97-27 | ND |
Burkholderia pseudomallei 1026b | ND |
Clostridium perfringens WAL-14572 | ND |
Escherichia coli EDL933 | ND |
Francisella tularensis subsp. tularensis SCHU S4 | ND |
Micrococcus luteus SK58 | ND |
Neisseria meningitidis 9506 | ND |
Pseudomonas sp. 2_1_26 | ND |
Salmonella enterica subsp. enterica LT2 | ND |
Staphylococcus aureus TCH1516 | ND |
Staphylococcus epidermidis SK135 | ND |
Streptococcus pneumoniae TCH8431 | ND |
Vibrio cholerae 395 | ND |
Yersinia pestis CO92 | ND |
Barcode Btk1 in Bacillus thuringiensis serovar kurstaki HD-1 | 23.927 |
Microbial Mock Community B (Even, High Concentration) | ND |
Microbial Mock Community B (Even, High Concentration) + Barcode Btk1 in Bacillus thuringiensis serovar kurstaki HD-1 | 23.725 |
Soil DNA extract | ND |
Soil DNA extract + Barcode Btk1 in Bacillus thuringiensis serovar kurstaki HD-1 | 23.969 |
Negative control (H2O) | ND |