Cancer immune control dynamics: a clinical data driven model of systemic immunity in patients with metastatic melanoma

Table 1 CICD data analysis for cancer patient 12A and healthy volunteer 10

BI-linear, modulated relationships				Cancer patient 12A		Healthy volunteer 10
Quad: Modulator, Source, Target, LSV
Triplet: Modulator, Source, Target
Doublet: Modulator, Source
Modulator	Source	Target	LSV	OCC. Count	Sum	OCC. count	Sum
IP-10	CD3+	CD3+	CD3+	100.00		100.00
IP-10	CD3+	CD3+			Triplet: 100		Triplet: 100
IP-10^a	CD3+^a	IL-8^a	IL-8^a	93.75		89.58
IP-10	CD3+	IL-8	EOTAXIN	35.42		2.08
IP-10	CD3+	IL-8	IP-10	29.17		2.08
IP-10	CD3+	IL-8	MCP-1	29.17		6.25
IP-10	CD3+	IL-8	VEGF	25.00		6.25
IP-10	CD3+	IL-8	TGFA	20.83		16.67
IP-10	CD3+	IL-8	IL-1A	20.83		8.33
IP-10	CD3+	IL-8	EGF	18.75		4.17
IP-10	CD3+	IL-8	IL-12P40	14.58		2.08
IP-10	CD3+	IL-8	FGF-2	14.58		2.08
IP-10	CD3+	IL-8	FRACTALKINE	14.58		4.17
IP-10	CD3+	IL-8	G-CSF	12.50		0.00
IP-10	CD3+	IL-8	IL-12P70	12.50		0.00
IP-10	CD3+	IL-8	IL-1RA	12.50		4.17
IP-10	CD3+	IL-8	IL-3	12.50		2.08
IP-10	CD3+	IL-8	MCP-3	12.50		2.08
IP-10	CD3+	IL-8	TNFB	12.50		2.08
IP-10	CD3+	IL-8	IFN-G	10.42		0.00
IP-10	CD3+	IL-8	IL-2	10.42		0.00
IP-10	CD3+	IL-8	MIP-1A	10.42		0.00
IP-10	CD3+	IL-8	GM-CSF	8.33		0.00
IP-10	CD3+	IL-8	IFN-2A	8.33		2.08
IP-10	CD3+	IL-8	IL-10	6.25		0.00
IP-10	CD3+	IL-8	IL-13	6.25		4.17
IP-10	CD3+	IL-8	IL-17A	6.25		0.00
IP-10	CD3+	IL-8	IL-1B	6.25		4.17
IP-10	CD3+	IL-8	IL-4	6.25		4.17
IP-10	CD3+	IL-8	IL-9	6.25		4.17
IP-10	CD3+	IL-8	MIP-1B	6.25		0.00
IP-10	CD3+	IL-8	TNFA	6.25		2.08
IP-10	CD3+	IL-8	FLT-3L	4.17		0.00
IP-10	CD3+	IL-8	IL-7	4.17		2.08
IP-10	CD3+	IL-8	IL-15	2.08		2.08
IP-10	CD3+	IL-8	IL-6	2.08		4.17
IP-10	CD3+	IL-8	IL-5	0.00		0.00
IP-10	CD3+	IL-8		502.08	Triplet: 502.08	183.33	Triplet: 183.33
IP-10	CD3+				Doublet: 11,839.58^b		Doublet: 5210.42^b

Linear, non-modulated relationships				Cancer patient 12A		Healthy volunteer 10
Quad: Modulator, Source, Target, LSV
Triplet: Modulator, Source, Target
Doublet: Modulator, Source
Modulator	Source	Target	LSV	OCC. Count	Sum	OCC. Count	Sum
n.a	IL-8	CD3/CD69+	CD3/CD69+	18.75	Triplet: 18.75	0.00	Triplet: 0
n.a	IL-8	CD11c/CD86+	CD11c/CD86+	10.42	Triplet: 10.42	0.00	Triplet: 0
n.a	IL-8	CD11c/HLA-DR+	CD11c/HLA-DR+	10.42	Triplet: 10.42	0.00	Triplet: 0
n.a	IL-8	CD11c+	CD11c+	8.33	Triplet: 8.33	0.00	Triplet: 0
n.a	IL-8	CD4/CD294+	CD4/CD294+	6.25	Triplet: 6.25	0.00	Triplet: 0
n.a	IL-8	CD4/TIM3+	CD4/TIM3+	6.25	Triplet: 6.25	0.00	Triplet: 0
n.a	IL-8	CD56+	CD56+	2.08	Triplet: 2.08	0.00	Triplet: 0
n.a	IL-8			83.33	Doublet: 83.33	0.00	Doublet: 0

43 of the 10,869 active Quad’s for the cancer patient and 25 of the 10,757 active Quad’s for the healthy volunteer are presented. Every row represents one unique active Quad containing the four components (LSV, Target, Source, and Modulator) and a value for its Occurrence Count. Examples of both bi-linear, modulated relationships (top section) and linear non-modulated relationships (bottom) are shown. The Triplets have the same Target, Source, and Modulator and its value is the sum of all its LSVs Occurrence Counts. The Doublets have the same Source and Modulator, combining all Target and LSV biomarkers. The value is the sum of all Occurrence Counts that have this common Doublet within its Quad.
^aDominator LSV, Target and LSV are the same cytokine in Quad
^bActual Doublet Value is higher due to additional Quads not shown

ISSN: 1471-2105