Fig. 4
From: PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
Overview of base editing input and output. a Segment of the BE specific user interface. A target sequence in text format can be provided by the user to install an edit. For correcting a specific SNV, genomic coordinates and the orientation of the gene must be provided by the user. C → T or G → A SNVs can be selected for correction. b Example ‘Single sample run’ for a random ClinVar variant. The target adenine is highlighted in red. Other adenines in the editing window that could be modified are in blue. Edit position relative to the PAM, PAM sequence and the corresponding ABE are shown. c Example base editing template for the ‘Multi sample run’