Fig. 3

Sub-repertoire detection across many samples. CDR3 clustering, and sub-repertoire detection using both hierarchical clustering (hc) and k-means (km) clustering of the CDR3 cluster centroids was performed for all 8 CD PBMC samples. a Shows proportions of sub-repertoires containing CDR3 cluster centroids from only n samples, dots are the estimate from each of 10 analyses from subsampled repertoires with sequencing depths of 1 to 10 thousand unique nucleotide CDR3s per sample. b The cumulative proportion of sub-repertoires containing representative clusters from n samples or more is shown; the cumulative at each n is computed as the mean proportion of n represented samples from the 10 resample analyses