Fig. 5From: Low-level variant calling for non-matched samples using a position-based and nucleotide-specific approachMinimum Variant Allele Fraction (VAF) for positions in PIK3CA with reference nucleotide cytosine required for Position-Based Variant Identification using the OverGrowth (PBVI-OG) model at 600× test sample depth. a Minimum VAF callable at every position where the reference nucleotide is cytosine and for each alternate nucleotide was calculated and plotted for the model. Blue (adenine), green (guanine), yellow (thymine). b Boxplot of minimum VAF detected for all nucleotide changesBack to article page