Fig. 3
From: Streamlining differential exon and 3′ UTR usage with diffUTR
Differential UTR analysis on real data. a. 3′ UTR lengthening during neuronal differentiation. Plotted are the UTR bins found statistically significant (bin- and gene-level FDR both < 0.1) by diffUTR (diffSplice2) when comparing in vitro differentiated neurons to mouse embryonic stem cells. The color indicates the point density. The clear skew towards a positive bin-level foldchange, especially for bins with a higher mean count (CPM = counts per million reads sequenced), is indicative of a UTR lengthening. b Receiver-operator characteristic (ROC) curves of differential UTR usage analysis on the LTP dataset, using 3′ sequencing to establish the ground truth. The axes are square-root-transformed to improve visibility, and only a subset of method variations are shown (see Additional file 1: Figure 1 for all variants)