Fig. 1

Overview of the experimental and analytical setup. a Workflow for expressing and visualizing variants via IF and automated widefield fluorescent microscopy. b MAPS software contains the following four modules: (1) image quality control (see Fig. 3); (2) cell detection (see Fig. 4); (3) phenotype discovery (see Figs. 5, 6); and (4) phenotype scoring (see Fig. 7). All figures are generated using Adobe Illustrator version 23.0.6