Fig. 7

Module 4: phenotype scoring identified loss of function PTEN variants. a Representative ROIs of the three PTEN localization classes that were used to train a classification model on Azure. b The performance metrics of the phenotype classification model for each class label. c Results of automated phenotype scoring for wildtype PTEN and 12 variants. d LOF scores for the same variants as in b taken from a previously published spheroid assay [21]. e Scatter plot showing the correlations between the LOF scores and the percentage of cells with nuclear PTEN. The best fit line is also shown