Fig. 3

Definition of true positive and false positive with respect to the tools reporting IS element’s fragments or ORFs. a Definition of true positive: A hit is considered as a true positive (TP) if it overlaps with any item in the reference dataset. If multiple hits overlap with the same IS element in the reference dataset, then all of these hits count as one hit only. b Definition of false positive: Each reported hit of the tool is matched against the database of known IS elements (ISfinder), and if several adjacent hits map to the same IS element at DNA or ORF level, then these hits are counted as only one merged FP