Fig. 3

Distribution of mean breadth of coverage across various target read depths and genome assembly versions. The y-axis represents the average breadth of coverage across 1KGP exomes, for each gene. Each boxplot represents the distribution across all CCDS genes at read depths from 5 × to 100x. At each read depth, the distributions of values from each genome assembly versions (GRCh37 and GRCh38) are shown as separate boxplots. The overall breadth of coverage levels of genes is highest at 5 × and decreases as read depths are increased. Given the same read depth level, little difference is observed between genome assembly versions. Lower tails of boxplots–especially at low read depths as 5 × or 10x–indicate genes with poor breadth of coverage in most of 1KGP exomes