Fig. 1

Demonstration of Segmentor software for nuclear refinement. a Raw microscopy volumes of the brain are loaded into the software. b Segmentor provides an initial segmentation of nuclei within the image (alternatively, pre-segmentations from other programs can be loaded). c The segmented images are manually refined within Segmentor using (1) the 3D visualization of segmented nuclei and (2) the 2D slices. (3) The region table enables the user to track progress during segmentation. d Finally, the manually refined image that can be used as gold standard input to deep learning programs is shown (grey regions indicate those the user has marked as completed). Image made in part using BioRender