Fig. 3
From: ORFik: a comprehensive R toolkit for the analysis of translation
Analysis of differential regulation. ORFik supports within-group (a) and between-group (b) plots for analyzing translational regulation. a TE vs mRNA levels using the average values across replicates. b Differential translation analysis between conditions. Non-significant genes are colored black while significant genes (p < 0.05) are grouped into 3 categories according to deltaTE classification [40]: Translational regulation — mRNA abundance is static while translation changes (orange), Buffering — mRNA and ribosome profiling regulated in opposite directions (purple), mRNA abundance regulation — significant change in mRNA abundance and a corresponding significant change in ribo-seq levels (green). Example data from two zebrafish developmental stages from Bazzini et al. 2014 (Additional file 1: Table S9), each with two replicates [46]