Fig. 2
From: MUREN: a robust and multi-reference approach of RNA-seq transcript normalization
Comparison of the log ratios of the ERCC sequences using M–A plots obtained by different methods. RNA-seq data are from THI experiments. The x-axis represents averaged normalized read counts (A-value). The y-axis represents log ratios (M-value). The ERCC sequence groups of different preset ratios are shown in four colors. Dots: log ratio estimate of individual ERCC sequence after normalization; dashed lines: fitted values of the dots by local smoothing (LOWESS); solid lines: nominal relationships between M- and A-values. The results of MUREN, UQ, TMM, and RLE are fair; Those of CPM and TPM are inadequate; Those of Q and RUV are distorted