Fig. 5
From: MUREN: a robust and multi-reference approach of RNA-seq transcript normalization
Cell cycle scRNA-seq data: the transition of phases is associated with the skewness of log ratios at both the single cell and the bulk level. AThe histograms of pairwise skewness of log ratios between cells at different phases. Left top: compared with cell in S phase, the cells in G1 phase are more active, in brief notation, G1 > S, and the transcriptomic differentiation is positively skewed; Right top: S > G2/M; left bottom: G2/M < G1; right bottom: G1/S > G2/M. Under the null hypothesis that the skewness is randomly positive or negative with equal probabilities, the nonparametric sign tests report extremely significant p-values. B Log ratios’ densities of pseudo bulk counts obtained by summing over cells in the same phase. After normalization by MUREN-sp, the modes of the densities of the pseudo bulk counts align to zero. The skewness of (normalized) bulk log ratios is consistent with that at the single cell level. Notice that the skewness of normalized and unnormalized log ratios has little difference