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Fig. 5 | BMC Bioinformatics

Fig. 5

From: CHIPIN: ChIP-seq inter-sample normalization based on signal invariance across transcriptionally constant genes

Fig. 5

Graphs generated by CHIPIN allowing evaluating specificity of two antibodies used: A antibody ab4729 (Abcam) against H3K27ac in an adrenocortical carcinoma sample (unpublished), and B antibody ACM39155 (Active Motif) against H3K27me3 in the shSpi1-A2B cells (unpublished). For ab4729, as expected, highly expressed genes show a higher level of H3K27ac than genes of medium or low expression; while for ACM39155, highly expressed genes show an increase of intensities for H3K27me3 downstream TSS (red). This suggests a potential non-specific binding of this antibody targeting H3K27 methylation to acetylated lysines, also documented by Rothbart et al. [20]

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BMC Bioinformatics

ISSN: 1471-2105

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