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Fig. 1 | BMC Bioinformatics

Fig. 1

From: A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells

Fig. 1

Automated high-content/high-resolution imaging workflow. A 96-well microplates were imaged using an automated spinning disk confocal microscope with a 63 × water immersion objective. B Total number of imaged fields (grid) present in a standard 0.32 cm2 well of a 96-well plate (yellow) including an imaged area of 7 × 7 fields. C Enlarged 7 × 7 imaged area containing U-2 OS cells stably expressing the ER marker Sec61β-mEmerald (green) and nuclei stained with Hoechst 33342 (blue). Scale bar = 200 μm. D Enlarged single imaging field. Scale bar = 50 μm. E Enlarged region of the imaged field showing the detail of the ER network obtained with the 63 × objective. Scale bar = 5 μm. F Images correspond to maximum intensity projection of 5 confocal planes interspaced at 0.5 μm intervals

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BMC Bioinformatics

ISSN: 1471-2105

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