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Fig. 3 | BMC Bioinformatics

Fig. 3

From: A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells

Fig. 3

Definition of SER polygon regions. A–D Sequential key steps in the analysis shown on the same U-2 OS cell and enlarged region of the cell. Scale bars = 20 μm and 5 μm on the enlarged images. A U-2 OS cell expressing the ER marker Sec61β-mEmerald (white). B Definition of the peripheral ring-shaped region of the cytoplasm region of interest (ROI). C Masking of ER tubules (green) applying a threshold to the Sec61β-mEmerald signal within the ROI. D Inversion of ER mask resulting in detection of internal polygonal regions (yellow) and areas between the ER and the detected plasma membrane (blue). E Steps A and D shown in a group of cells. Scale bar = 50 μm

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BMC Bioinformatics

ISSN: 1471-2105

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