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Figure 1. | BMC Bioinformatics

Figure 1.

From: shinyCurves, a shiny web application to analyse multisource qPCR amplification data: a COVID-19 case study

Figure 1.

An overview example of two shinyCurves analyses (intercalating dye and fluorescent probe, in green and pink, respectively). Both analyses can be performed using qRT-PCR results from BioRad CFX or Applied Biosystems Quant Studio platforms, among others. The input data required for each analysis are specified in the black boxes. In green, the intercalating dye pipeline starts with the plotting of melting curves to discard unspecific amplification products. Then, in the calling analysis each sample is assigned a final result (Positive, Negative, Undetermined). In pink, the fluorescent probe pipeline starts with the calling analysis and is followed by the plotting of amplification curves for the visual inspection of the results

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