Fig. 4
From: FASTAFS: file system virtualisation of random access compressed FASTA files
Screenshot showing compatibility with ’samtools view’ for CRAM files. The right panel shows an active fastafs mount process with debug messages enabled, mounting the hg19 FASTAFS archive to ./mnt_hg19. The left panel shows three samtools operations: Command 1 shows the original SAM-file. Command 2 runs the samtools conversion from SAM to CRAM, requiring the mounted FASTA file by the mount process (right panel). Command 3 shows the contents of the CRAM file. The CRAM file lacks actual sequence data, which samtools needs to extract from the FASTA file mounted in the right panel. The FASTAFS mount process (right) shows that samtools has requested access to both ./mnt_hg19/hg19.fa and ./mnt_hg19/hg19.fa.fai