Fig. 6

Spatial confinement during ribosome specialization: a test case of the COSNet\(_i\) workflow. Optimized conditions were used to test whether the distribution of substoichiometric proteins is significantly constrained to specific ribosomal regions in yeast and mammalian systems. The weighed graph used to select regions was optimized as detailed in Fig. 5. The code commands used to produce our results are outlined in Code Chunk 1. The mammalian and yeast systems were tested and only the yeast depleted substoichiometric rProteins were significantly localized in a SSU region (colored in yellow shades) after Bonferroni correction of the Fisher exact test p-values (i.e., Region 2: P = 0.00004, Padj = 0.0005). The mRNA has been colored red to outline its relative location as compared to the region enriched in depleted proteins. Ribosomal structures are rotated 90\(^{\circ }\) in the y-axis at a time in order to visualize the boundaries of the significantly changed region