Fig. 3

Depicting relationships of HMM Scores. Whole sequence scores are represented in blue and HMM domain scores are represented in red. Sets of sequences excluded from the creation of each HMM profile were used to define the limits of detection, represented by a grey line. Datasets of 404 RIFIN-A, 476 RIFIN-B, and 40 STEVOR sequences excluded from the HMM training were used to test and define the limits of detection for each profile. All RIFIN-A and -B sequences had low concordance to the STEVOR profile, failing to meet its threshold score of 145. The 404 RIFIN-A sequences had whole sequence (blue) and domain (red) scores that exceeded the thresholds for the RIFIN-A profile. In contrast, none of the 404 RIFIN-A sequences met classification criteria for RIFIN-Bs, as their domain scores (red) were below the threshold score of 250. The Y-axis represents HMM scores, and the X-axis represents the ordered numerical label for each sequence