| | RIFIN (185) | Non-RIFIN | Predictive value | | STEVOR (43) | Non-STEVOR | Predictive value |
---|
STRIDE | Called + | 182 | 0 | 100% | Called + | 38 | 0 | 100% |
| Called − | 3 | 5363 | 100% | Called − | 5 | 5505 | 99.9% |
| | 98.4% | 100% | | | 88.3% | 100% | |
PFAM | Called + | 178 | 0 | 99% | Called + | 38 | 0 | 100% |
| Called − | 7 | 5363 | 99.8% | Called − | 5 | 5505 | 99.9% |
| | 96.2% | 99.9% | | | 88.3% | 100% | |
TIGRFAM | Called + | 177 | 0 | 100% | Called + | 38 | 0 | 100% |
| Called − | 8 | 5363 | 99.8% | Called − | 5 | 5505 | 99.9% |
| | 95.70% | 100% | | | 88.3% | 100% | |
- #We made comparisons across tools using the same parameters as STRIDE
- ^The curated 3D7 reference genome served as a gold standard. There are a total of 5548 sequences in the P. falciparum 3D7 reference, where 182 sequences are annotated as RIFINs and 43 sequences are annotated as STEVORs (includes 27 RIFIN and 10 STEVOR pseudogenes). Unlike PFAM and TIGRFAM, STRIDE was not trained with the entire 3D7 RIFIN and STEVOR repertoire (Fig. 2). The bolded text illustrates the sensitivity of each program; all three tools had 100% specificity