Table 1 Example for tab-delimited table structure serving IMDA as input adapted from the barcode.txt file of the provided test data. The format is analog to the one used by MIGEC. This file should contain for each sample: a sample ID defining the name of the sample and the barcode sequence containing the barcode (here: CAGAT) and optional UMI (represented by “N”). Further, if available, an additional barcode sequence can be defined. Mandatory inputs are the FASTQ files containing all sequencing reads, forward (#1) and reverse (#2)
#Sample ID | Master barcode sequence (barcode and UMI) | Additional barcode sequence | FASTQ #1 | FASTQ #2 |
|---|---|---|---|---|
1_A_nS_r1 | NNNNNNtCAGATtNNNNNNtcttgggg | idx1_R1_001.fastq.gz | idx1_R2_001.fastq.gz | |
1_A_nS_r2 | NNNNNNtCAGATtNNNNNNtcttgggg | idx2_R1_001.fastq.gz | idx2_R2_001.fastq.gz | |
2_A_nS_r1 | .. | .. | .. | |
2_A_nS_r2 | . | . | . |