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Fig. 4 | BMC Bioinformatics

Fig. 4

From: GRAde: a long-read sequencing approach to efficiently identifying the CYP11B1/CYP11B2 chimeric form in patients with glucocorticoid-remediable aldosteronism

Fig. 4

A case in which the cross-over region was validated by Sanger sequence analysis. A Long-range PCR revealed PCR products of the CYP11B1/CYP11B2 chimeric gene in sample #17. Amplification with a chimeric gene is expected to produce a 3.9 kb product. B The fusion plot of sample #17 reveals a possible cross-over region located in intron 2, with high background noise. C Sequencing of the chimeric PCR product demonstrated that the crossover site was located in intron 2. The nucleotides that differed between CYP11B1, CYP11B2, and the chimeric gene are highlighted in different colors

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BMC Bioinformatics

ISSN: 1471-2105

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