Fig. 6

Recapitulating previously identified cell populations using CellSeg. A. Visualization of identified populations in a representative CRC tissue. Points on scatter plots show positions of cells on the displayed tissue image in Fig. 5B. Population identity obtained through gating. B. Fluorescent image of a representative CRC tissue. Expression of six phenotyping markers used in Fig. 5A shown. C. Population correlation analysis between CellSeg and WTS. Each point corresponds to a TMA spot, where the X value is the gated population count from WTS and the Y value is the count computed from CellSeg. Least-squares regression line displayed along with r² value. T cells are defined as CD45⁺CD3⁺CD20⁻Cytokeratin⁻; macrophages as CD45⁺CD20⁻CD3⁻ and CD68⁺, CD163⁺, or CD68⁺CD163⁺; B cells as CD45⁺CD20⁺CD3⁻Cytokeratin⁻; and tumor as Cytokeratin⁺CD45⁻