Fig. 2

Strandedness proportions in RNA-Seq data. Strandedness proportions were evaluated for 20 studies for each h. sapiens, s. cerevisiae, and a. thaliana using how_are_we_stranded_here and varying the number of input reads sampled. Results are not included where zero reads were psuedoaligned, and triangles denote results where the proportion of reads psuedoaligned is less than 0.1. Studies for which the strandedness proportion was between 0.6 and 0.9 (dashed lines), and those which do not match the reported strandedness are highlighted. how_are_we_stranded_here was run using the full Ensembl cDNA annotation for each species