Fig. 4
Detection level and Performance evaluation of PΨFinder. A Detection level based on sequencing depth. Samples with an average coverage of 144X or more (gray dashed line) are confident PΨg-insertion site predictions as determined by downsampling analysis (blue triangles, Additional file 3: Table S4). Samples with an average coverage of 95 or less (red dashed line) are below the detection level of PΨFinder. Samples between these thresholds are likely to be true predictions and manual inspection of the results is required. Biological samples are shown as red circles. B Performance evaluation. A total of 117 samples were analyzed. TP, true positives; TN, true negatives; FP, false positives; FN, false negatives; F1 score, harmonic mean of the precision and sensitivity; FDR, false discovery rate; TPR, true positive rate; PPV, positive prediction value