Fig. 1

Steps of data analysis using ScreenGarden. The ScreenGarden application offers a tool for stepwise analysis of plate-based high-throughput screens and was specifically adapted to facilitate the analysis of screens with one or two independent controls. The ‘Calculate-LGRs’ script performs statistical analysis of colony sizes compared to a single control. After statistical analysis, the data can be downloaded as a ‘mean file’, which contains the average data of replicates, or as a ‘replicates file’, which contains all data of individual controls. The ‘mean file’, or a ‘merge file’ which is generated by combining the datasets of two independent controls, can be uploaded and plotted for quality control and data visualisation using the ‘Plots’ tool. In addition, the user can choose to define cut-off thresholds using the ‘Mixture Model’ tool