Skip to main content
Fig. 3 | BMC Bioinformatics

Fig. 3

From: ScreenGarden: a shinyR application for fast and easy analysis of plate-based high-throughput screens

Fig. 3

Combining analyses with two independent controls using ScreenGarden. A The raw data (plate images) from two control plates to the same experimental plate are shown together for one 1536 density plate with four replicates per strain. Control-specific hits are highlighted in red (control 1) and blue (control 2) boxes respectively. B The LGR values for comparing the experiment independently with each control are plotted. The dashed line visualises the empirical cut-off value of LGR ≥ 0.4 for the average LGRs of both control comparisons. C These data are shown as a Venn diagram, with the dashed circle indicating all data with an average LGR (of the two controls) ≥ 0.4. Using two controls rather than one defines a subset of high-confidence growth defects and excludes control-specific effects

Back to article page