Table 1 Nearest-neighbor parameters for DNA/DNA duplexes
From: CROPSR: an automated platform for complex genome-wide CRISPR gRNA design and validation
Nearest-neighbor sequence | \(\Delta G^\circ _{37}\) |
|---|---|
\((5^{\prime } \rightarrow 3^{\prime }{/} 3^{\prime }\rightarrow 5^{\prime })\) | (kJ × mol−1 |
AA/TT | −4.26 |
AT/TA | −3.67 |
AC/TG | −6.09 |
AG/TC | −5.40 |
TA/AT | −2.50 |
TC/AG | −5.51 |
TG/AC | −6.12 |
CG/GC | −9.07 |
GC/CG | −9.36 |
CC/GG | −7.66 |
Terminal A/T | 4.31 |
Terminal C/G | 4.05 |