Fig. 3

Variance of allele balance calculated for isolates of B. lactucae previously confirmed as homokaryotic or heterokaryotic. Whole-genome sequencing datasets of thirty isolates that had been sequenced to high coverage were downsampled to 10× to the highest coverage of factor by tens up to 100×. a Allele frequency plots of isolates previously identified as homokaryotic (SF5) or heterokaryotic (all others). At 10× coverage it is not possible to distinguish the homokaryotic isolate from the heterokaryons. At higher coverage, multiple allele peaks are evident corresponding to different numbers of genotypes. b Variance of allele balance was calculated from the same downsampled datasets. At 10×, homokaryotic and heterokaryotic isolates could be differentiated from one another. The difference in variance between the two groups increased as sequencing coverage increased. Smoothing through kernel density estimation was used to identify contours around the datasets. Labels indicate isolates presented in A. See the Results section for discussion of isolates 1689 and 1806 that appear different compared to other heterokaryotic isolates, but do not fit the expectations for diploidy at any coverage